A study on relationship between the expression of CDR gene in Candida albicans and fluconazole susceptibility test in vitro / 预防医学

ABSTRACT

Objective ToinvestigatethefluconazoleresistanceofclinicalstrainsofCandidaalbicansandtoexplorethe relationshipbetweenclinicaldrugresistance(CDR)geneofCandidaalbicansandfluconazoleresistance.Methods Using the M27-A2 micro broth dilution method,221 strains of Candida albicans from sputum separation,interrupt urine,feces and throat swabs which conserved in our laboratory during the year 2010 were measured with fluconazole MIC value. Candida albicans 18S RNA was used as internal reference,the transcription levels of CDR1 and CDR2 gene in resistant and sensitive strain groups were compared under different fluconazole concentrations with RT-PCR technique.Results Amongtherandomlyselected221strainsofCandidaalbicans,127(57.47%)strainsweresusceptibletofluconazole,24 (10.86%)strains were sensitive dependence on dose and 70 (31.67%) strains were resistance.Under different concentration,the expression of CDR1 and CDR2 in resistant strain group was significantly higher than that of sensitive straingroup(P<0.05).Conclusion ThefluconazoleresistantrateofCandidaalbicansisrelativehighandthe expression of CDR1 and CDR2 in resistant strain group is higher than that of sensitive strain group.