Effect of Shenling Baizhu San on Murine Model of IBD Induced by DSS in Mice Through Regulating of Autophagy / 中国实验方剂学杂志

ABSTRACT

Objective:To investigate the effect of Shenling Baizhu San on inflammatory bowel disease (IBD) induced by 5% dextran sodium sulfate (DSS) in mice by regulating autophagy of intestinal epithelial cells. Method:The 84 BALB/c mice were randomly divided into 7 groups with 12 mice in each group. In addition to the normal group, 5% DSS was freely drunk for 7 days to induce acute inflammatory bowel disease. In the treatment group, high, medium and low doses of shenlingbaishu powder (12,6,3 g·kg-1·d-1), mesalazine (2 g·kg-1·d-1) and autophagy inducer rapamycin (4 mg·kg-1·d-1) were given by gavage. Hematoxylin-eosin (HE) staining was used to observe the morphological changes of colon tissues in mice. The autophagosome formation of intestinal epithelial cells was detected by transmission electron microscopy. Western blot was used to detect the ratio of autophagy related protein LC3-Ⅱ/LC3-Ⅰ, phosphatidylinositol-3kinase (PI3K), mammalian target of rapamycin (mTOR), ubiquitin-binding protein-1 (p62), Beclin1 phosphorylation, ULK1, 4EBP protein expression. Result:Compared with normal group, model group mice colonic mucosa epithelial cells are widely missed, most incomplete glands, change in colitis, LC3-Ⅱ content decreased significantly (PPPPPConclusion:The effect of Shenling Baizhu San on DSS-induced IBD is related to the regulation of the phosphorylation of PI3K, mTOR and p62 proteins in the autophagy pathway of intestinal epithelial cells.