Crebanine inhibits voltage-dependent Na+ current in guinea-pig ventricular myocytes / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 20-23, 2014.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-812314
Responsible library:
WPRO
ABSTRACT
AIM@#To study the effects of crebanine on voltage-gated Na(+) channels in cardiac tissues.@*METHODS@#Single ventricular myocytes were enzymatically dissociated from adult guinea-pig heart. Voltage-dependent Na(+) current was recorded using the whole cell voltage-clamp technique.@*RESULTS@#Crebanine reversibly inhibited Na(+) current with an IC50 value of 0.283 mmol·L(-1) (95% confidence range 0.248-0.318 mmol·L(-1)). Crebanine at 0.262 mmol·L(-1) caused a negative shift (about 12 mV) in the voltage-dependence of steady-state inactivation of Na(+) current, and retarded its recovery from inactivation, but did not affect its activation curve.@*CONCLUSION@#In addition to blocking other voltage-gated ion channels, crebanine blocked Na(+) channels in guinea-pig ventricular myocytes. Crebanine acted as an inactivation stabilizer of Na(+) channels in cardiac tissues.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Aporphines
/
Pharmacology
/
Drugs, Chinese Herbal
/
Down-Regulation
/
Cells, Cultured
/
Chemistry
/
Cell Biology
/
Myocytes, Cardiac
/
Stephania
/
Voltage-Gated Sodium Channels
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2014
Document type:
Article