Pleurotus nebrodensis polysaccharide (PN-S) enhances the immunity of immunosuppressed mice / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 760-766, 2015.
Article
in En
| WPRIM
| ID: wpr-812485
Responsible library:
WPRO
ABSTRACT
In the present study, the effects of Pleurotus nebrodensis polysaccharide (PN-S) on the immune functions of immunosuppressed mice were determined. The immunosuppressed mouse model was established by treating the mice with cyclophosphamide (40 mg/kg/2d, CY) through intraperitoneal injection. The results showed that PN-S administration significantly reversed the CY-induced weight loss, increased the thymic and splenic indices, and promoted proliferation of T lymphocyte, B lymphocyte, and macrophages. PN-S also enhanced the activity of natural killer cells and increased the immunoglobulin M (IgM) and immunoglobulin G (IgG) levels in the serum. In addition, PN-S treatment significantly increased the phagocytic activity of mouse peritoneal macrophages. PN-S also increased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), and nitric oxide (NOS) in splenocytes. qRT-PCR results also indicated that PN-S increased the mRNA expression of IL-6, TNF-α, INF-γ, and nitric oxide synthase (iNOS) in the splenocytes. These results suggest that PN-S treatment enhances the immune function of immunosuppressed mice. This study may provide a basis for the application of this fungus in adjacent immunopotentiating therapy against cancer and in the treatment of chemotherapy-induced immunosuppression.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Phagocytosis
/
Pharmacology
/
Polysaccharides
/
Biological Products
/
Cell Line
/
Chemistry
/
Immunosuppression Therapy
/
Interleukin-6
/
Interferon-gamma
/
Tumor Necrosis Factor-alpha
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2015
Document type:
Article