Anti-proliferative and apoptotic effects of S1, a tetrandrine derivative, in human gastric cancer BGC-823 cells / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 527-533, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-812594
Responsible library:
WPRO
ABSTRACT
The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. The anti-proliferative activity was determined by MTT assay; the induction of cell cycle arrest and apoptosis were detected by flow cytometry. Quantitative real time RT-PCR and Western blotting were used to evaluate the mRNA and protein expression levels in mitochondrial pathway. S1 significantly reduced cell viability and induced a G2/M phase arrest and apoptosis in dose- and time-dependent manner. Further studies showed that S1 increased mRNA and protein expression of Bax and the Bax/Bcl-2 ratio. Moreover, S1 decreased the protein expression of procaspase-9 and procaspase-3, suggesting that the induction of apoptosis may be related to the alteration of the ratio of Bax/Bcl-2 and the activation of caspases. These findings suggested that S1 merits further investigation as a novel therapeutic agent for the treatment of human gastric cancer.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Stomach Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Stomach Neoplasms
/
Cell Survival
/
Chemistry
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Line, Tumor
/
Benzylisoquinolines
/
Cell Proliferation
/
Drug Therapy
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2016
Document type:
Article