Treatment with total hepatic vascular exclusion and reperfusion for the intestinal barrier in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 433-436, 2005.
Article
in Zh
| WPRIM
| ID: wpr-813541
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the influence of treatment with total hepatic vascular exclusion and reperfusion on the intestinal barrier in rats.@*METHODS@#The total hepatic vascular exclusion and reperfusion model was built after the block of hepatic portal, suprahepatic and infraheptic vena cava for 20 minutes. Sixty Sprague-Dawley rats were divided randomly into 2 groups: sham operation group (Group A, n=30) and total hepatic vascular exclusion and reperfusion treatment group (Group B, n=30). Each group was subdivided randomly into 3 subgroups (n=10) according to different experiment time points as follows: at the end of the total hepatic vascular exclusion (T0), 4 reperfusion after total hepatic vascular exclusion (T1) and the 48 h survival. Portal vein blood gas was analysed at T0. At T0 and T1 the following items were detected: the level of portal vein blood D-lactate, tumor necrosis factor-alpha (TNF-alpha), the MDA concentration and pathologic morphology change of intestinal mucosa.@*RESULTS@#Compared with Group A, the PCO2 at T0 in Group B increased while pH, P02, and HCO3- decreased significantly (P < 0.05). The level of portal blood D-lactate, TNF-alpha and intestinal mucosa MDA at T0 and T1 was significantly higher (P < 0.05, or P < 0.01). The histologic damage in the intestinal mucosa was observed in Group B, and the rat survival in Group B was lower than that in Group A (P < 0.05).@*CONCLUSION@#The treatment with total hepatic vascular exclusion and reperfusion can damage the intestinal barrier in rats.
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Reperfusion Injury
/
Random Allocation
/
Rats, Sprague-Dawley
/
Bacterial Translocation
/
Intestinal Mucosa
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Ischemia
/
Liver
/
Microbiology
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2005
Document type:
Article