Porcine endogenous retrovirus in Daweizi pigs in Hunan / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 838-842, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-813590
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To detect porcine endogenous retrovirus (PERV) in Daweizi pigs and to provide basic parameters of evaluating the biological safety for xenotransplantation from pigs to humans.@*METHODS@#Ear tissues from 42 individuals were randomly collected from a Daweizi pig population. PCR and RT-PCR were performed to detect PERV proviral DNA and mRNA respectively. Finally, env-A, env-B, and env-C were amplified, sequenced, and analyzed using the BLAST software in National Center for Biotechnology Information.@*RESULTS@#PERV proviral DNA and mRNA could be detected in the 42 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were detected in all the individuals. Compared with other pig species (AY288779, DQ011794 and AY534304), there was 1 and 8 bp differences in the sequences of env-A and env-C, while no difference in env-B.@*CONCLUSION@#PERV exists and has transcriptive activity in Daweizi pigs. The predominate subtype is PERV-ABC. Env genes are firstly cloned and sequenced in Daweizi pigs and there are polymorphism in the breed. As to the biological safety, the breed was not suitable as a donor in xenotransplantation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Swine
/
Transplantation, Heterologous
/
Virology
/
DNA, Viral
/
Polymerase Chain Reaction
/
Endogenous Retroviruses
/
Methods
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2006
Document type:
Article