Killing effect of VP3 on human nasopharyngeal carcinoma cell line CNE-2 cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 706-709, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-813616
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the killing effects of VP(3) on nasopharyngeal carcinoma cell line CNE-2.@*METHODS@#Plasmid expression vector pcDNA3.1(-) CMV.VP(3)-His was constructed and identified by Kpn I/EcoR I endonuclease analysis, and then sequenced to verify successful insertion in the sense direction of VP(3) gene. pcDNA3.1(-) CMV.VP(3)-His and pcDNA3.1(-)-His expression plasmid was transiently transfected into nasopharyngeal carcinoma cell line CNE-2 . VP(3) protein expression was detected by Western blotting. MTT assay was used to determine the killing effects of VP(3) gene on nasopharyngeal carcinoma cell line CNE-2.@*RESULTS@#Endonuclease analysis and sequencing confirmed the recombinant plasmid contained the complete VP(3) CDS sequence. Western blotting detected a 14.03 kD protein expression from the transfected cells, which was the expecting band of VP(3) gene. The growth of CNE-2 cells that expressed VP(3) gene was inhibited,while the growth of CNE-2 cells that did not express VP(3) gene was not inhibited.@*CONCLUSION@#VP(3) gene can kill nasopharyngeal carcinoma cell CNE-2.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Molecular Sequence Data
/
Base Sequence
/
Transfection
/
Genetic Therapy
/
Nasopharyngeal Neoplasms
/
Capsid Proteins
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2006
Document type:
Article