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Effect of precursor of brain-derived neurotrophic factor on hippocampal neuron survival and its mechanism / 中南大学学报(医学版)
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-813796
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of precursor of brain-derived neurotrophic factor (proBDNF) on cultured hippocampal neuron and its intracellular mechanism.@*METHODS@#The hippocampal neurons were dissociated from E18 rats and cultured in neurobasal medium, and then the cells were treated with proBDNF, preBDNF(propeptide of proBDNF) and proBDNF antiserum,respectively. Thirty minutes, 1 hour or 48 hours later, the cells were stained with Nissl solution, and the immunocytochemistry methods of ELK-p (Ets domain protein), ErK2(extracellular signal regulated kinase) and c-fos were performed.@*RESULTS@#The expressions of ELK-p, ErK and c-fos were significantly upregulated in the cultured hippocampal neurons after they were treated with proBDNF protein,and the immuno-positive staining was also obvious in some nuclei. While the endogenous proBDNF was neutralized by proBDNF antiserum treatment, the expressions of ELK-p, ErK and c-fos were downregulated and many cells showed swelling and vasoculation. The immunoreactivity in preBDNF treated cells was similar to that in normal cultured cells.@*CONCLUSION@#proBDNF plays an important role in sustaining the hippocampal neuron survival through upregulating the ELK and ErK pathways.
Subject(s)
Full text: Available Database: WPRIM (Western Pacific) Main subject: Pharmacology / Protein Precursors / Signal Transduction / Cell Survival / Cells, Cultured / Proto-Oncogene Proteins c-fos / Brain-Derived Neurotrophic Factor / Mitogen-Activated Protein Kinase 1 / Cell Biology / Proto-Oncogene Proteins c-ets Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2007 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Main subject: Pharmacology / Protein Precursors / Signal Transduction / Cell Survival / Cells, Cultured / Proto-Oncogene Proteins c-fos / Brain-Derived Neurotrophic Factor / Mitogen-Activated Protein Kinase 1 / Cell Biology / Proto-Oncogene Proteins c-ets Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2007 Document type: Article
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