Proteomic study of paclitaxel on human cervical carcinoma HCE1 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 512-517, 2008.
Article
in Zh
| WPRIM
| ID: wpr-814047
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the mechanism of paclitaxel on the protein expression of human cervical carcinoma cell line HCE1.@*METHODS@#The total proteins extracted from paclitaxel-treated HCE1 cells were analyzed by 2-dimensional gel electrophoresis (2-DE), and compared with those from untreated HCE1 cells. The differential proteins were identified by mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.@*RESULTS@#At 24 hour after paclitaxel (0.05 mumol/L) treatment, 2-DE images of paclitaxel-treated and paclitaxel-untreated cells were analyzed. Forty-two differential proteins were found. Twenty-one differential proteins among 42 proteins were analyzed by mass spectrometry, among which 15 proteins were identified, including peptidyl-prolylisomerases A (PPIase A),alpha-enolase,keratin 8,heat shock protein 90, eukaryotic translation initiation factor 1A, and so on.@*CONCLUSION@#Fifteen proteins in human cervical carcinoma cells paclitaxel-treated and paclitaxel-untreated are found by proteomic techniques. These proteins may be involved in the proliferation inhibition of human cervical carcinoma cells by paclitaxel.
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Phosphopyruvate Hydratase
/
Tumor Cells, Cultured
/
Biomarkers, Tumor
/
Eukaryotic Initiation Factor-1
/
Uterine Cervical Neoplasms
/
Genome
/
Paclitaxel
/
Proteome
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Document type:
Article