Change of vWF, PAl-1 and t-PA in rats with hyperlipemia and its significance / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 415-420, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-814062
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish the hyperlipoidemia model and observe its effect on the expression of plasma vWF, PAl-1 and t-PA after endothelial cell injury caused by the model at different time in rats.@*METHODS@#Sixty male Sprague-Dawley rats weighing (200+/-20)g were randomly divided into 6 groups (n=10 in each) high flat composition for 7 weeks (Group A7), 8 weeks (Group A8)and 9 weeks(Group A9), common bait vessel feeding for 7 weeks(Group C7), 8 weeks(Group C8) and 9 weeks (Group C9). When it reached the setting time, the rats were put to death and the serum was separated to detect the blood-fat immediately. Meanwhile, the pathological sections of the rat aorta were made to observe the endothelial injury. The blood plasma was separated and the content of the blood plasma PAl-1, t-PA and vWF was detected by ELISA assay.@*RESULTS@#(1)TG, TC and LDL of the rats intragastrically administrated high flat composition were higher than those of the control group,and HDL increased with time at first, but lowered when longer than 9 weeks. (2) There were no lesions in the aortic endothelium in all rats of Group C through optical microscope,while different lesions in the aortic endothelium in all rats of Group A were observed. (3)In the 3 groups A, the plasma vWF and PAI-1 were gradually increasing with time while t-PA gradually decreased. (4) There was no significant difference of vWF between Group A7 and Group A8. PAI-I increased gradually with the aggravation of endotheliocytes in Group A; and t-PA decreased gradually with the aggravation of endothelium in Group A.@*CONCLUSION@#(1) As intragastric administration prolongs, the plasma lipid of rats keeps increasing with the aggravation of the endodermis injury. (2) PAI-1, t-PA and vWF are the molecular markers of vascular endothelial cell injury in rats. (3) PAI-l and t-PA are more sensitive than vWF in reflecting the endothelial cell injury in hyperlipemia early metaphase.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Endocrine System Diseases
Database:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pathology
/
Blood
/
Von Willebrand Factor
/
Random Allocation
/
Tissue Plasminogen Activator
/
Rats, Sprague-Dawley
/
Plasminogen Activator Inhibitor 1
/
Endothelial Cells
/
Hyperlipidemias
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Document type:
Article