Expression of caveolin-1 and the invasion of choriocarcinoma / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 331-337, 2008.
Article
in Zh
| WPRIM
| ID: wpr-814074
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the association between the expression of caveolin-1(CAV-1) and the invasion of choriocarcinoma, and to explore the effect of CAV-1 small interfering RNA(siRNA) on the invasion of choriocarcinoma cell line JEG-3.@*METHODS@#(1) Matrigel invasion assay and 3-(4,4)-dimethylthiahiazo (-z-yl)-3,5-di-phenytetrazoliumormide (MTT) assay were used to examine the difference in invasion and proliferation ability between JEG-3 cells and JAR cells;(2) Expression of caveolin-1 gene in the human chorionic villi tissues and chorionicnoma cell lines (JEG-3 cells and JAR cells) were detected by semi-quantitative RT-PCR. (3) The effect of CAV-1 siRNA transfection on the expression of CAV-1 mRNA, and the invasion and proliferation ability of JEG-3 cells were measured by RT-PCR, Matrigel invasion assay and MTT assay.@*RESULTS@#(1) The invasion ability of JEG-3 cell line was stronger than that of JAR cell line (P0.05);(2) The expression of caveolin-1 gene in chorionicnoma cell lines was significantly stronger than that in the human normal chorion(P<0.05), and the expression of caveolin-1 gene in JEG-3 cells was stronger than that in the JAR cells (P<0.05). The data suggested that there was significantly positive correlation between caveolin-1 and the invasiveness of chorionicnoma cells (r=0.086,P<0.05);(3) CAV-1 siRNA could knock-out the expression of CAV-1 mRNA, and inhibit the invasion and proliferation ability of chorionicnoma cells.@*CONCLUSION@#CAV-1 can promote the invasion ability of chorionicnoma cells. CAV-1 siRNA can inhibit the invasion and proliferation ability of chorionicnoma cells.
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Uterine Neoplasms
/
RNA, Messenger
/
Tumor Cells, Cultured
/
Choriocarcinoma
/
RNA, Small Interfering
/
RNA Interference
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Caveolin 1
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Genetics
/
Metabolism
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Document type:
Article