Expression of EBI3 and p28 mRNA in the brain and spinal cord of chronic model of experimental autoimmune encephalomyelitis in C57BL/6J mice / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1028-1036, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-814157
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the expression of EBI3 and p28 mRNA (the 2 subunits of IL-27) in the brain and spinal cord of the model of experimental autoimmune encephalomyelitis (EAE), and to explore their effect on EAE.@*METHODS@#Seventy-two adult female SPF C57BL/6J mice (inbred strain) were randomly divided into a control group, an adjuvant group, and an EAE group. RT-PCR was performed to detect the expression of EBI3 mRNA and p28 mRNA in the brain and spinal cord.@*RESULTS@#The expression of EBI3 mRNA and p28 mRNA was up-regulated at onset in the EAE group, which increased quickly during peak phase and maintained at a high level in the chronic phase. There was significant difference in the expression of EBI3 and p28 mRNA between the EAE group and the control/adjuvant group (P0.05).@*CONCLUSION@#IL-27 may play a role of promoting the morbility of EAE in the early stage, and sustain the inflammatory response in endgame.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Spinal Cord
/
Brain
/
RNA, Messenger
/
Minor Histocompatibility Antigens
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Chronic Disease
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Interleukins
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Receptors, Cytokine
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Protein Subunits
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Encephalomyelitis, Autoimmune, Experimental
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Document type:
Article