Preventive effect of exogenous hydrogen sulfide on hepatic fibrosis in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 911-915, 2012.
Article
in Zh
| WPRIM
| ID: wpr-814766
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To determine the effect of exogenous hydrogen sulfide on experimental hepatic fibrosis in rats and its mechanism.@*METHODS@#Wistar male rats were randomly divided into three groups: a normal control group (n=8), a model group (n=8), and a hydrogen sulfide prevention group (n=8). The rat model of hepatic fibrosis was reduced by intraperitoneal injection of carbon tetrachloride (CCl4). The prevention group, in addition to intraperitoneal injection of 40% CCl4, was intraperitoneally administered H2S once a day until 8th week. After the experiment, the liver function and liver fibrosis were assayed. Liver tissue samples were used for histopathological changes. The expression of TGF-β1 in liver tissue was detected by RT-PCR and Western blot.@*RESULTS@#Compared with the model group, the levels of ALT, AST, HA, LN, and PC III in the sulfide group were significantly reduced (P<0.01 or P<0.05), ALB content was increased (P<0.05) in the serum, TGF-β1 expression was obviously reduced, and the degree of liver fibrosis was improved (P<0.05).@*CONCLUSION@#Exogenous hydrogen sulfide can effectively inhibit the development of hepatic fibrosis, reduce the expression of TGF-β1, and decrease the the sediment of extracellular matrix in the liver tissues.
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Carbon Tetrachloride
/
Rats, Wistar
/
Therapeutic Uses
/
Extracellular Matrix
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Transforming Growth Factor beta1
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Hydrogen Sulfide
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Liver
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Liver Cirrhosis
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2012
Document type:
Article