GPI-PLD inhibits the growth of hepatoma cells by down-regulation of PI3K-Akt signaling pathway / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 873-878, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-815509
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To clarify the effect of glycosylphosphatidylinositol-specific phospholipase D (GPIPLD) on hepatoma cells HepG2 and the possible molecular mechanism.@*METHODS@#MTT, fluorescent staining and Western blot were applied to analyze the effect and molecular mechanism of GPI-PLD on hepatoma cells by transfected high expression GPI-PLD model. We inoculated HepG2 in nude mice models to further clarify the effect of GPI-PLD on hepatoma cells in vivo.@*RESULTS@#Compared with the control groups, PI3K-Akt signaling pathway activity and proliferation of hepatoma cells were significantly inhibited in the GPI-PLD group. Nude mice models showed that the tumor growth and tumor weight [(1.87 ± 0.09) g] of the GPI-PLD group were significantly less than those of the blank control group [(2.20 ± 0.17) g] and the negative control group [(2.15 ± 0.09) g]. AST, ALT and AFP serum concentration in the GPI-PLD group were significantly lower than those of the control groups (P<0.05).@*CONCLUSION@#GPI-PLD can inhibit the proliferation of hepatoma cells and growth in vivo, and promote the apoptosis of hepatoma cells by reducing the activity of PI3K-Akt signaling pathway.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Liver Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Phospholipase D
/
Transfection
/
Signal Transduction
/
Down-Regulation
/
Cell Line
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Phosphatidylinositol 3-Kinases
/
Proto-Oncogene Proteins c-akt
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2014
Document type:
Article