Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen / 대한임상미생물학회지
Annals of Clinical Microbiology
; : 21-31, 2020.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-816605
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium caprae and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen.METHODS:
Of 310 MTC isolates obtained from clinical samples in a tertiary care hospital from February 2017 to August 2018, MolecuTech Real TB-Taq (YD Diagnostics, Korea) real-time PCR was performed, specifically to detect MTB. For DNA showing MTB negative results by MTB-specific real-time PCR or pyrazinamide-resistant strains, PCR-based MTC typing, spoligotyping, and exact tandem repeat D gene sequencing were performed.RESULTS:
All the 310 MTC isolates were identified to be MTB. Two MTB strains of East-African-Indian 4-Vietnam genotype, which have not been reported in Korea, were also found.CONCLUSION:
There was no zoonotic tuberculosis in this study. Since we investigated only 310 MTC isolates detected in only one medical institution, multi-center study is needed to accurately know the prevalence of zoonotic tuberculosis in Korea.
Full text:
Available
Health context:
SDG3 - Health and Well-Being
/
Neglected Diseases
/
SDG3 - Target 3.3 End transmission of communicable diseases
Health problem:
Target 3.3: End transmission of communicable diseases
/
Neglected Diseases
/
Tuberculosis
/
Zoonoses
/
Tuberculosis
Database:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Tertiary Healthcare
/
DNA
/
Goats
/
Prevalence
/
Infection Control
/
Sequence Analysis
/
Tandem Repeat Sequences
/
Real-Time Polymerase Chain Reaction
/
Genotype
Type of study:
Controlled clinical trial
/
Prevalence study
Country/Region as subject:
Asia
Language:
Korean
Journal:
Annals of Clinical Microbiology
Year:
2020
Document type:
Article