Simultaneous Determination of Isoquercitrin ，Astragalin and Salvianolic Acid B in Moringa oleifera Leaves Granules by HPLC / 中国药房

ABSTRACT

OBJECTIVE： To establish a method for simultaneous determination of isoquercitrin， astragalin and salvianolic acid B in Moringa oleifera leaves granules. METHODS： HPLC method was adopted. The determination was performed on Cosmosil-C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at flow rate of 1.3 mL/min. The column temperature was 40 ℃ and detection wavelength was set at 260 nm. The sample size was 10 μL. RESULTS： The linear ranges of isoquercitrin， astragalin and salvianolic acid B were 0.017-0.341， 0.010-0.194， 0.010-0.195 mg/mL， respectively （all r＞0.999 0）. The detection limits were 0.085， 0.143， 0.117 μg/mL， and the limits of quantitation were 0.283， 0.476， 0.392 μg/mL， respectively. RSDs of precision， stability （24 h） and reproducibility tests were all lower than 2.0％ （n＝6）. Average recoveries were 101.22％， 98.76％ and 98.72％， and RSDs were 0.66％，0.30％，0.30％ （n＝6）， respectively. CONCLUSIONS： The established method is simple， accurate and reproducible. It can be used for simultaneous determination of isoquercitrin， astragalin and salvianolic acid B in M. oleifera leaves granules.