In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum
Asian Pacific Journal of Tropical Medicine
; (12): 103-106, 2012.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-819819
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To identify the possible antiplasmodial compounds from leaf, stem, root and flower extracts of Ocimum canum (O. canum), Ocimum sanctum (O. sanctum) and Ocimum basilicum (O. basilicum).@*METHODS@#The O. canum, O. sanctum and O. basilicum were collected from Ramanathapuram District, Tamil Nadu and the extraction was carried out in ethanol. The filter sterilized extracts (100, 50, 25, 12.5, 6.25 and 3.125 μg/mL) of leaf, stem, root and flower extracts of O. canum, O. sanctum and O. basilicum were tested for antiplasmodial activity against Plasmodium falciparum (P. falciparum). The potential extracts were also tested for their phytochemical constituents.@*RESULTS@#The leaf extract of O. sanctum showed excellent antiplasmodial activity (IC(50) 35.58 μg/mL) followed by leaf extract of O. basilicum (IC(50) 43.81 μg/mL). The leaf extract of O. canum, root extracts of O. sanctum and O. basilicum, the stem and flower extracts of all the three tested Ocimum species showed IC(50) values between 50 and 100 μg/mL. Statistical analysis reveals that, significant antiplasmodial activity (P <0.01) was observed between the concentrations and time of exposure. The chemical injury to erythrocytes was also carried out and it shows that, there were no morphological changes in erythrocytes by the ethanolic extract of O. canum, O. sanctum and O. basilicum. The in vitro antiplasmodial activity might be due to the presence of alkaloids, glycosides, flavonoids, phenols, saponins, triterpenoids, proteins, resins, steroids and tannins in the ethanolic extracts of tested plants.@*CONCLUSIONS@#The ethanolic leaf extracts of O. sanctum possess lead compounds for the development of antiplasmodial drugs.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Malaria
/
Neglected Diseases
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Plasmodium falciparum
/
Solvents
/
Plant Extracts
/
Chemistry
/
Malaria, Falciparum
/
Plant Leaves
/
Plant Bark
/
Ocimum
Limits:
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2012
Document type:
Article