Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix metalloproteinase-9 expression
Asian Pacific Journal of Tropical Medicine
; (12): 482-485, 2011.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-820107
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the molecular mechanisms of antitumor properties of triptolide, a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F.@*METHODS@#Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h. Then the expression and activity of matrix metalloproteinase (MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9-treated HT-1080 cells.@*RESULTS@#18 nmol/L triptolide inhibited the gene expression and activity of MMP-9, but not those of MMP-2, in HT-1080 cells. In addition, both 18 nmol/L triptolide and 3 μg/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells, by about 50% and 35%, respectively, compared with the control. Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells.@*CONCLUSIONS@#These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Phenanthrenes
/
Down-Regulation
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 9
/
Cell Line, Tumor
/
Diterpenes
/
Drug Therapy
/
Epoxy Compounds
Limits:
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2011
Document type:
Article