Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells
Asian Pacific Journal of Tropical Medicine
; (12): 659-662, 2014.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-820636
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60.@*METHODS@#50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 15 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.@*RESULTS@#Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.@*CONCLUSIONS@#Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Physiology
/
Dendritic Cells
/
Leukemia
/
Cell Differentiation
/
Cytokines
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Apoptosis
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HL-60 Cells
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Coculture Techniques
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Cell Biology
Limits:
Female
/
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2014
Document type:
Article