CD34, VEGF and endothelin-1 expression in intrahepatic metastasis of hepatocellular carcinoma / 中华普通外科杂志
Chinese Journal of General Surgery
; (12): 932-935, 2019.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-824736
Responsible library:
WPRO
ABSTRACT
Objective To explore the roles of CD34,VEGF and endothelin-1 in hepatic sinusoid capillarization and the intrahepatic metastasis of hepatocellular carcinoma.Methods Data of 10 cases of hepatocellular carcinoma without intrahepatic metastasis were compared with those 10 cases of hepatocellular carcinoma with intrahepatic metastasis.Liver cancer tissues and paracancerous tissues were collected,hepatic sinusoidal capillarization was detected by immunohistochemistry,mRNA expression and protein expression of CD34,VEGF and endothelin-1 genes were detected by QPCR,Western blot,and the relationship between gene expression and metastasis of HCC was observed.Results Hepatic sinusoid capillarization was observed in carcinoma tissues,not in the adjacent tissues,whereas mRNAs and proteins of CD34,VEGF and endothelin-1 were higher in liver cancer tissues than that in the adjacent tissuess.In addition,both mRNA and protein expression levels of these genes were significantly higher in the intrahepatic metastatic liver cancers than those without metastasis (P < 0.05).Conclusion CD34,VEGF and endothelin-1 may play important roles in intrahepatic metastasis of liver cancer.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of General Surgery
Year:
2019
Document type:
Article