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miR-497 over-expression inhibits epithelial-mesenchymal transition in lung cancer A549 cells by targeting cyclin E1 / 中国肿瘤生物治疗杂志
Article in Zh | WPRIM | ID: wpr-831392
Responsible library: WPRO
ABSTRACT
@#[摘 要] 目的:探讨过表达miR-497靶向细胞周期蛋白E1(cyclin E1,CCNE1)对肺癌A549细胞上皮间质转化(epithelial-mesenchymal transition,EMT)的影响。方法:常规培养人肺癌A549细胞,细胞实验分为正常组(不加干预)、对照组(转染miR-497 mimics-NC)、实验组(转染miR-497 mimics)。采用Transwell小室实验、免疫荧光染色、qPCR、Western blotting法分别检测各组细胞迁移和侵袭能力、蛋白间质标志物α-SMA和上皮标志物E-cadherin的表达、miR-497和CCNE1的表达水平,荧光素酶基因基因报告实验验证miR-497和CCNE1的靶向关系。结果:与对照组和正常组相比,实验组A549细胞迁移和侵袭的数量明显减少(均P<0.05),细胞的间质标志物α-SMA的绿色荧光强度明显减弱[(36.95±5.81) vs (98.69±2.36)、(97.94±2.63),均P<0.05],上皮标志物E-cadherin的绿色荧光强度明显增强[(388.41±10.93) vs (100.95±6.37)、(102.55±3.18),均P<0.05],miR-497的表达明显升高(均P<0.05),CCNE1的表达均明显下降(均P<0.05)。miR-497能够靶向调控CCNE1的表达。结论:在肺癌A549细胞中miR-497能够靶向调控CCNE1的表达,上调miR-497的表达后能明显抑制A549细胞迁移和侵袭能力,影响EMT相关蛋白的表达。
Key words
Full text: 1 Database: WPRIM Language: Zh Journal: Chinese Journal of Cancer Biotherapy Year: 2020 Document type: Article
Full text: 1 Database: WPRIM Language: Zh Journal: Chinese Journal of Cancer Biotherapy Year: 2020 Document type: Article