HPLC for simultaneous determination of naringin, hesperidin, neohesperidin, acteoside and liquiritin in Jinlongshe oral solution / 第二军医大学学报

ABSTRACT

Objective To establish an HPLC method for simultaneous determination of five effective components (naringin, hesperidin, neohesperidin, acteoside and liquiritin) in the Jinlongshe oral solution. Methods SunFire™ C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was acetonitrile (B) and 0.2% formic acid (A), with gradient elution program as follow 0-10 min, 10%-19% B; 10-32 min, 19% B. The flow rate was 1.0 mL/min, and the temperature was at 40℃. The injection volume was 10 μL, and the detection wavelength was set at 285 nm. Results There were good linearities for concentrations of naringin, hesperidin, neohesperidin, acteoside and liquiritin within the range of 2.00-60.00, 2.12-63.60, 1.68-50.40, 2.00-60.00 and 2.00-60.00 μg/mL (r≥0.999 9), respectively. RSD of the intra-day and inter-day precisions of the five components were less than 2.30% and 3.82%, respectively. RSD of the stability in 24 h was less than 4.47%. The average recoveries were 95.11%, 93.73%, 96.39%, 98.02% and 95.12%, with the RSD being 2.08%, 4.05%, 4.02%, 6.01% and 4.56%, respectively. Conclusion The present method has been proven to be convenient, accurate, sensitive and with good reproducibility, and can be applied for the quality control and assessment of Jinlongshe oral solution.