High mobility group box chromosomal protein 1 promotes IL-18 production and IL-18 receptor expression in macrophages / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 1353-1357, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-840418
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate whether high mobility group box chromosomal protein 1 (HMGB1) can activate macrophages to secrete IL-18.Methods:
Mouse peritoneal macrophages were cultured with HMGB1 for indicated period, then the mRNA and protein expression of IL-18 and IL-18 receptors were examined by real-time PCR, ELISA, and flow cytometric analysis. Toll-like receptor (TLR) 2 and 4 agonists (1 μg/ml Pam3Cys or 100 ng/ml LPS) were used as parallel controls.Results:
Two hours after culture with HMGB1(100 ng/ml), IL-18 mRNA expression was greatly increased. The expression of IL-18 protein was also greatly increased 24 h after culture with HMGB1, and the expression of IL-18R was slightly up-regulated. Pam3Cys (1 μg/ml) and LPS(100 ng/ml) could promote the expression of IL-18 at both mRNA and protein levels in the macrophages, and could up-regulate IL-18 receptors.Conclusion:
HMGB1 can directly activate macrophages to secrete IL-18 and to express IL-18 receptor, suggesting that IL-18 may mediate the proinflammatory activity of HMGB1.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Document type:
Article