Screening for molecular markers for early diagnosis of lung cancer by bacteriphage display / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 1157-1161, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-840727
Responsible library:
WPRO
ABSTRACT
Objective:
To establish a lung cancer phage-peptide library and to screen for biomarkers for early detection of lung cancer.Methods:
A phage display library was constructed using 30 lung-cancer tissue samples from Changhai Hospital. Protein-A/G agarose was used to enrich IgG from control sera as well as lung cancer sera. Five biopannings were carried out for enrichment of lung cancer-specific phage clones. Five hundred phage clones were randomly selected,those with D>2.0(lung cancer plasma pool v. s. control plasma pool) were selected for DNA sequencing and protein prediction.Results:
(1) The recombination rate of the phage library was 60%,with the average phage titer being 3.0 × 106 pfu and a volume being 9.0 × 106 pfu. (2) Of 19 phage colonies selected by ELISA and sequenced,9 were cancer-related genes,8 with unknown function, and 2 were not related to cancer.Conclusion:
The screened genes in the phage colonies might serve as biomarkers for the early diagnosis of lung cancer.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Document type:
Article