Obestatin inhibits proliferation and differentiation of 3T3-L1 preadipocytes / 第二军医大学学报

ABSTRACT

Objective: To investigate the inhibitory effects of obestatin on proliferation and differentiation of 3T3-L1 preadipocytes. Methods: Obestatin (10-8 mmol/L, 10-9 mmul/L, 10-10 mmol/L, 10-11 mmol/L, and 10-12 mmol/L) and ghrelin (10-10 mmol/L) were used to treat 3T3-L1 preadipocytes. Cell proliferation was assessed by MTT assay and the results were compared with that of blank control group. The differentiation of 3T3-L1 preadipocytes (from day 1 to day 10) was interfered with obestatin or ghrelin (both at 10-10 mmol/L). Intracellular fat accumulation in differentiated adipocytes was determined by oil red O staining and the expression of PPAR-γ2 mRNA was detected by RT-PCR; the results were compared with that of control group (induced with routine inducer). Results: Compared with the blank control group, obestatin-treated groups (various concentrations of obestatin) had significantly less cells(P<0.05). Oil red O staining revealed that, compared with control group, the formation of lipid droplets was significantly decreased after 10 days' of treatment with 10-10 mmol/L obestatin (P<0.05). The expression of PPARγ2 gene increased with the progress of 3T3-L1 preadipocytes differentiation. PPAR-γ2 mRNA level in mature adipocytes of obestatin group was significantly lower than that in the cells of control group. The effect of ghrelin was contrary to that of obestatin. Conclusion: Obestatin can inhibit the proliferation and differentiation of 3T3-L1 preadipocyes.