Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois / 대한구강보건학회지

ABSTRACT

OBJECTIVES: This study aims to identify and partially purify antibacterial compounds against Streptococcus mutans from Galla Rhois extract. METHODS: Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper discdiffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). RESULTS: The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using partition chromatography and HPLC, and the antibacterial activity was confirmed. CONCLUSIONS: The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.