A compound staining method showing collagenic, elastic fibers and melanocyte in skin tissues / 第二军医大学学报

ABSTRACT

Objective: To develop a compound staining method showing collagenic, elastic fibers and melanocyte in skin tissues. Methods: A staining meth od combining Masson Fontana silver nitrate, Ponceau S, Picric acid and Victoria blue (MFSN-P-PA-VB) was used in the present animal skin experimental. Results: The melanin granule was blac k in the skin melanocyte, collagenic fibers were red, the red blood cells at hemorrhagic focus were yellow, and the elastic fibers were green by this MFSN-P-PA-VB staining method. Conclusion: This compound staining method has multicol or and fine contrast, and can better show melanins, the red blood cells at hemorrhagic focus, and collagenic and elastic fibers.