Fingerprint analysis of codonopsis radix by HPLC coupled with chemometrics analysis / 中草药·英文版

ABSTRACT

Objective: To establish a validated high performance liquid chromatography (HPLC) for the fingerprint analysis of Codonopsis Radix and for the determination of lobetyolin. Methods: HPLC coupled with diode array detection method was employed to establish the fingerprint profile and quantitative determination of lobetyolin in Codonopsis Radix. Principal component analysis method was employed to analyze the 52 Codonopsis Radix samples. Results: The reference chromatogram was generated with 25 common peaks showing good separation from adjacent peaks. Conclusion: Statistical analysis of the obtained data demonstrates that the developed HPLC fingerprint combined with chemometric is a reliable method for the similar evaluation and quality assessment of Codonopsis Radix, and other traditional Chinese herbs. © 2013 Tianjin Press of Chinese Herbal Medicines.