HPLC fingerprint for quality control of Ramulus Cinnamomi / 国际药学研究杂志

ABSTRACT

Objective To establish HPLC fingerprint for the quality control of Ramulus Cinnamomi. Methods The HPLC fingerprint was obtained with Diamonsil C18 column(4. 6 mm × 200 mm,5 μLm)and a gradient elution with the mobile phase consisting of acetonitrile and glacial acetic acid-water (0. 04: 99. 96) at flow rate of 1. 0 ml/min. The detector wavelength was set at 270 nm while the njection volume was 20 μl. The HPLC generated chromatographic data were analyzed using a similarity evaluation computer program and hierarchical cluster analysis (HCA). Results 9 common peaks were detected. The results of similarity analysis and HCA ndicated that the samples from different places can be divided nto two categories: one had a higher content of cinnamic acid, which plays a significant role n pharmacologic action of Ramulus Cinnamomi, while the other contained ess of it. Conclusion The method of HPLC fingerprint established n this experiment is rapid and efficient. It is an effective method for the quality control of Ramulus Cinnamomi.