D3 and D5 Dopamine Receptor mRNA Expression in Peripheral Blood Mononuclear Cells from Patients with Parkinson's Disease
Journal of the Korean Neurological Association
; : 359-364, 1999.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-8478
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Among 5 subfamilies of dopamine receptors (DAR), D3 and D5 DAR are expressed on peripheral blood mononuclear cells (PBMC). Recently, those DARs have been reported to change in Parkinson's disease (PD).METHODS:
We measured the DAR mRNA expression in PBMC from 15 PD patients who had never taken antiparkinson medication, and 16 age-matched healthy people by reverse transcription and quantitative competitive polymerase chain reaction. The beta-actin mRNA expression was also measured to evaluate the relative expression of DAR mRNA.RESULTS:
The D3 and D5 DAR mRNA expression was not different between patients and controls. In patients, no significant cor-relation was found between DAR mRNA expression in PBMC and clinical variables such as severity and duration of symptoms, and patients' age.CONCLUSIONS:
We confirmed the presence of D3 and D5 DAR in PBMC. However, their mRNA expressions were not influenced by the disease process of PD.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Parkinson Disease
/
RNA, Messenger
/
Dopamine
/
Polymerase Chain Reaction
/
Actins
/
Receptors, Dopamine
/
Reverse Transcription
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Neurological Association
Year:
1999
Document type:
Article