Effects of connective tissue growth factor on proliferation and migration of human osteosarcoma 143B cells in simulated microenvironment in vitro / 肿瘤

ABSTRACT

Objective: To investigate the effects of connective tissue growth factor (CTGF) on proliferation and migration of human osteosarcoma 143B cells in simulated osteosarcoma microenvironment by coculture technique in vitro, and to explore their underlying mechanisms. Methods: The osteosarcoma 143B cells were infected with recombinant adenovirus AdCTGF, then an indirect co-culture system was established with bone marrow stromal HS-5 cells for 3 days. The proliferation capacity of 143B cells was detected by MTT, and the migration ability of 143B cells was determined by wound-healing test and Transwell assay. The expression levels of CTGF and β-catenin were examined by reverse transcription-PCR (RT-PCR) and Western blotting. The expression levels of phosphorylated Akt (p-Akt) and phosphorylated glycogen synthase kinase 3β (p-GSK3β) were examined by Western blotting. Results: After infection with recombinant adenovirus AdCTGF, CTGF was overexpressed in osteosarcoma 143B cells in co-culture system. In simulated osteosarcoma microenvironment in vitro, CTGF overexpression could significantly promote the proliferation of 143B cells (P < 0.01), and the wound-healing rate and the number of migratory 143B cells also remarkably increased (both P < 0.01). The expression levels of P-catenin, p-Akt and p-GSK3β increased significantly in 143B cells after overexpressing CTGF (all P < 0.05). Conclusion: CTGF can significantly promote the proliferation and migration of human osteosarcoma 143B cells in simulated osteosarcoma microenvironment. PI3K (phosphatidylinositol 3-kinase)/Akt and Wnt/β-catenin pathways may be involved in this process.