Characteristics of PLA2 activity and expression of PLA2 subtypes in rat pancreas after simple pancreatic trauma / 解放军医学杂志

ABSTRACT

Objective To study the characteristics of phospholipase A2 (PLA2) activity and expression of PLA2 subtypes in rat serum and pancreas tissue with an established simple pancreatic trauma model in rats. Methods One hundred and twenty Wistar rats were divided into two groups in a one-to-two ratio control group and impact group. The two groups were subsequently divided into four subgroups at 6h, 24h, 72h, 7d time point. For the animals in the impact groups, the pancreas was injured by a BIM- III biotical impact machine, and the animals in control group were sham injured. The total catalytic activity of PLA2 in serum and pancreatic tissue, the expressions of mRNA and protein and immunohistochemical localization of secretory PLA2 (sPLA2), cytosolic PLA2 (cPLA2) and calcium independent PLA2 (iPLA2) in pancreatic tissue were determined after the corresponding pretreatments in each group. Results The total catalytic activity of PLA2 in serum and pancreatic tissue of impact groups increased at 6h after injury, and the PLA2 activity reached peak value at 24h in pancreatic tissue and at 72h in serum after trauma, and at 7d in serum and pancreatic tissue were still higher than that in control group at the same time (P<0.05). The results of RT-PCR, Western blotting and immunohistochemistry indicated that the expression of sPLA2 in pancreatic tissue was higher than that of cPLA2 and iPLA2. Conclusions PLA2 activity can be upregulated in serum and pancreas after pancreatic trauma. The expression of sPLA2 is increased most significantly in pancreatic tissue after pancreatic trauma. PLA2 activity may be detected in serum 6h-7d after pancreatic trauma, and is helpful for auxiliary diagnosis of simple pancreatic trauma.