Sesquiterpenoids from Artemisia integrifolia / 中草药

ABSTRACT

Objective To study the chemical constituents of Artemisia integrifolia. Methods The chemical constituents were separated and purified by column chromatographies and HPLC. Their structures were determined by spectroscopic analyses. Guaiane-type sesquiterpenoids were tested for their anticancer activity against uterine cancer (HeLa) by MTT method. Results Ten compounds were isolated from A. integrifolia with the structures identified as 3-methoxy-tanapartholide (1), dehydroleucodin (2), 4β-hydroxyguaia-2,10(14),11(13)-trien-12,6α-olide (3), 4α-hydroxyguaia-2,10(14),11(13)-trien-12,6α-olide (4), 2β-hydroxyguaia- 3,10(14),11(13)-trien-12,6α-olide (5), eartanomalide (6), 5-epi-seco-tanapartholide A (7), seco-tanapartholide B (8), iso-seco- tanapartholide (9), and 3-epi-iso-seco-tanapartholide (10). Conclusion All compounds are isolated from this plant for the first time. The results showed that all sesquiterpenoids had potent inhibiting effects on HeLa cells proliferation. The inhibitory effects of compounds 1, 4, and 7 were the highest (IC50 < 7.8 μg/mL).