Simultaneous determination of 11 components in Naofuqing Capsule by HPLC-MS/MS / 中草药

ABSTRACT

Objective: To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination of 11 components including eleutheroside E, 2,3,5,4’-tetera-hydroxystilbene-2-O-β-D-glucoside (stilbene glycoside), isofraxidin, rosmarinic acid, notoginsenoside R1, salvianolic acid B, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rd, and emodin in Naofuqing Capsule. Methods: The analysis was performed on an Agilent Poroshell 120 EC-C18 column (75 mm × 4.6 mm, 2.7 μm), and the mobile phase consisted of water containing 0.1% formic acid and acetonitrile with gradient elution at the flow rate of 0.4 mL/min. The column temperature was maintained at 35 ℃. The MS detection for the 11 tested components was performed in negative ion multiple reaction monitoring mode. Results: Good linear relationship were observed in the test range for 11 components, and the recoveries of eleutheroside E, stilbene glycoside, isofraxidin, rosmarinic acid, notoginsenoside R1, salvianolic acid B, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rd, and emodin were 106.4%, 104.2%, 99.9%, 102.3%, 104.1%, 98.3%, 108.9%, 103.6%, 105.8%, 101.9%, and 104.2%, respectively. The contents of 10 batches of the 11 components were in the ranges of 0.274-0.310, 1.579-1.642, 0.093-0.099, 0.331-0.352, 1.115-1.229, 1.663-1.870, 4.884-5.173, 0.691-0.762, 2.974-3.358, 1.053-1.493, and 0.100-0.115 mg/g, respectively. Conclusion: The established method is sensitive, stable, and rapid. It is suitable for the simultaneous determination of multiple components in Naofuqing Capsule.