Chemical constituents from rhizomes of Cimicifuga dahurica / 中草药

ABSTRACT

Objective To study the chemical constituents from the rhizomes of Cimicifuga dahurica. Methods The chemical constituents from the rhizomes of C. dahurica in ethyl acetate extraction phase and H2O fraction were isolated and purified by chromatographic methods, such as silica gel, opening ODS column, Sephadex LH-20, ODS and semi-preparative HPLC. Which stucture were identified by NMR and physicochemical analysis. Results Fifteen compounds were isolated and identified as 20(R),23 (R),24(R),25(S),26(S)-16β23; 2326; 2425-triepoxy-12β-acetoxy-3β,26-dihydroxy-9,19-cyclolanost-7-ene-3-O-β- D-xylopyranoside (1), cimiaceroside B (2), 23-O-acetylshengmanol-3-O-β-D-xylopyranoside (3), 7,8-didehydro-24-O-acetylhydroshengmanol-3-O-β-D- xylopyranoside (4), 24-epi-cimigenol-3-O-β-D-xylopyranoside (5), 24-O-acetyldahurinol-3-O-β-D-xylopyranoside (6), 24-epi-24-O- acetylhydroshengmanol-3-O-β-D-xylopyranoside (7), 12β-O-acetylcimigenol-3-O-β-D-xylopyranoside (8), 23-O-acetyl-7,8- didehydroshengmanol-3-O-β-D-xylopyranoside (9), 7,8-didehydro-25-dehydrocimigenol-3-O-β-D-xylopyranoside (10), 25-O- ethylcimigenol-3-O-β-D-xylopyranoside (11), 3,4-dihydroxybenzoic acid (12), 24-O-acetylisodahurinol-3-O-α-L-arabinopyranoside (13), 2,3-dihydroxy-2-[(4-hydroxyphenyl) methyl]-1-ethyl ester (14), and 2,3-dihydroxy-2-[(4-hudroxyphenyl) methyl]-4-ethyl ester (15). Conclusion Compounds 6, 11-12, and 14-15 are isolated from Cimicifuga genus for the first time. Compounds 1, 4-5, 8-10, and 13 are isolated from C. dahurica for the first time.