HPLC fingerprint of Homalomena occulta / 中草药

ABSTRACT

Objective: To establish an HPLC method for the fingerprint analysis of Homalomena occulta and evaluate the quality though similarity calculation. Methods: Waters e2695 was applied to analyze ten batches of H. occulta. Phenomenex Gemini 110A C18 column was performed (250 mm × 4.6 mm, 5 μm) with acetonitrile and 0.01% phosphoric acid in agradient mode at a flow rate of 1.0 mL/min at 290 nm and the separation was performed at 30 ℃. The similarity was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A. Results: The HPLC characteristic fingerprint of H. occulta with 11 commons peaks, and one of them were identified by comparing with reference subslances. The values of similarity was higher than 0.887. The HPLC method showed better results of stability, precision, and repeatability. Conclusion: It is the first time to establish the HPLC fingerprint of H. occulta. Those described can not only provides an identification method for fingerprint, but also provides the foundation of quality control.