HPLC fingerprint identification of Lonicera Japonica Flos and Lonicera Flos / 中草药
Chinese Traditional and Herbal Drugs
; (24): 773-776, 2017.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-852985
Responsible library:
WPRO
ABSTRACT
Objective:
To compare the differences between Lonicera Japonica Flos and Lonicera Flos by establishing HPLC fingerprint and calculating the similarity.Methods:
The columns was Phenomenex Luna 5 μm C18 (2) 100 A, 250 mm×4.6 mm; The column temperature was 40℃. The mobile phase was acetonitrile-0.5% phosphoric acid, the flow rate was 1 mL/min, and the wavelength was 350 nm.Results:
HPLC fingerprint of Lonicera Japonica and similarity evaluation by screening large peak integration were established. The similarity of 12 batches of Lonicera Japonica Flos were all above 0.95, and four batches of Lonicera Flos were less than 0.80.Conclusion:
HPLC fingerprint profiles under 350 nm can reflex the differences between Lonicera Japonica Flos and Lonicera Flos effectively; Similarity evaluation by screening large peak integration shows the tiny differences of chemical component.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2017
Document type:
Article