Establishment of cell suspension culture system of Carthamus tinctorius and analysis of compounds by UPLC-Q-TOF/MS / 中草药

ABSTRACT

Objective: To study the establishment of cell suspension culture system and build a platform for exploring the functional genes in the biosynthesis pathway for Carthamus tinctorius. Methods: Using the cotyledon of C. tinctorius as explants for callus induction, callus with good quality was selected for suspension cultivation. The factors influencing the establishment of C. tinctorius cell suspension culture system were investigated, such as hormone, inoculum amount, pH, and cell activity. The effect of MeJA at different concentration on suspension cell growth rate was studied. Chemical compounds in suspension cell system were preliminary identified by UPLC-Q-TOF/MS. Results: The optimal medium for the cell suspension culture was MS + 1.0 mg/LTDZ + 0.1 mg/L NAA, the pH was 5.5-6.0, and inoculum amount was 0.02 g/mL. MeJA at different concentration had effects on suspension cell growth rate 50 μmol/L MeJA increased cell growth rate, 500 μmol/L MeJA depressed cell growth rate greatly. Thirteen potential chemical compounds in suspension cell system were preliminary identified. Conclusion: The cell suspension culture system of C. tinctorius is established, and MeJA with optimal concentration has effects on suspension cell growth rate.