Chemical constituents isolated from fruits of Cnidium monnieri and their effects on proliferation of UMR106 cells / 中草药

ABSTRACT

Objective: To study the chemical constituents from the fruits of Cnidium monnieri and their effects on proliferation of UMR106 cells. Methods: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. The proliferation of all isolated compounds on osteoblast-like UMR106 cells was determined. Results: Nine compounds were isolated and identified as 5,7-dihydroxy-6,8-dimethoxy-2-methyl-4H-chromen-4-one (1), 5-hydroxy-2- hydroxymethyl-4H-chromen-4-one (2), (+)-marmesin (3), bergaptol (4), isobergapten (5), 7-hydroxy-8-(2',3'-dihydroxy-3'-methyl-butyl)-coumarin (6), murraol (7), coniferyl aldehyde (8), and m-hydroxybenzoic acid (9). Compounds 1, 3, and 7 showed the significant proliferative activities on UMR106 cells lines at the concentration of 1 × 10-10 mol/L and the proliferative ratios were 31.55%, 32.39% and 30.87%. Conclusion: Compounds 1-6 are isolated from the specie of Cnidium Cusson for the first time. Compounds 1, 3, and 7 increase UMR106 cells proliferation to some extent.