HPLC fingerprints of crude and processed Ligustri Lucidi Fructus / 中草药

ABSTRACT

Objective: To set up an analysis method of chromatographic fingerprint for crude and processed Ligustri Lucidi Fructus by HPLC. Methods: Purospher® STAR RP18 Endcapped column (250 mm×4.6 mm, 5 μm) was used for separation. The separation process was carried out using a gradient elution, which was based on a mobile phase of the mixture aqueous solution with water-acetonitrile at the flow rate of 1.0 mL/min. The detection wavelength was 282 nm and the column temperature was 25℃. The chromatographic fingerprints were analyzed with the Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012. 130723 Edition). Results: Totally 19 common fingerprint peaks and five constituents including salidroside, privet glycosides, especially privet glycoside, oleuropein, and oleonuezhenide were identified after optimization of the detection wavelength, mobile phase, and gradient elution conditions. Furthermore, the similarity of HPLC fingerprints of 10 batches of Ligustri Lucidi Fructus was high, being over 0.9. Conclusion: The present methodology meets the technical requirements of fingerprint with the rapid, simple, and accuracy properties and could be a basis for the intrinsic quality control standard for Ligustri Lucidi Fructus.