Determination of salidroside and crenulatin in Rhodiolae Sacrae Herbra by HPLC-ELSD / 中草药
Chinese Traditional and Herbal Drugs
; (24): 1157-1159, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-854618
Responsible library:
WPRO
ABSTRACT
Objective:
To establish a determination method for the contents of salidroside and crenulatin in Rhodiolae Sacrae Herbra, and to compare the content differences of the two compounds in Rhodiolae Sacrae Herbra from different batches.Methods:
Using HPLC-ELSD method and Kromasil C18 column (250 mm × 4.6 mm, 5 μm), with acetonitrile and water as mobile phase, gradient elution at flow rate of 1.0 mL/min, column temperature of 30°C, nitrogen flow rate of 2.0 L/min, drift tube temperature of 45°C, and gain of 2.Results:
The linear of salidroside was 0.806-8.06 μg (r = 0.9996, n = 6), and the average recovery rate was 100.56%. The linear of crenulatin was 2.142-21.42 μg (r = 0.9999, n = 6), and the average recovery rate was 100.40%.Conclusion:
This method is simple, and accurate, and has better reproducibility for the determination of the two compounds in Rhodiolae Sacrae Herbra. It could be used as a quantitative determination method for salidroside and crenulatin in Rhodiolae Sacrae Herbra.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Document type:
Article