Helicobacter pylori Eradication Downregulates Cellular Inhibitor of Apoptosis Protein 2 in Gastric Carcinogenesis
Gut and Liver
; : 79-86, 2017.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-85473
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS:
To evaluate the expression of cellular inhibitor of apoptosis protein 2 (cIAP2) during gastric carcinogenesis after Helicobacter pylori (HP) infection and after HP eradication.METHODS:
We divided non-cancer patients into four groups according to the status of HP infection and atrophic gastritis (AG)/intestinal metaplasia (IM). We compared cIAP2 mRNA expression among these four groups and patients with HP-positive early gastric cancer (EGC) by using real-time polymerase chain reaction (PCR). We evaluated the expression of cIAP2 messenger RNA (mRNA)/protein by using real-time PCR/immunohistochemistry and the degree of apoptosis with a terminal deoxynucleotidyl transferase-mediated nick end labeling assay before and 12 months after endoscopic submucosal dissection (ESD) in HP-positive EGC patients, regardless of whether they had undergone eradication therapy.RESULTS:
The expression of cIAP2 mRNA was significantly higher in the groups with HP(+), AG/IM(+), and HP-positive EGC than in the control, HP(+), and AG/IM(−) groups (p<0.005). In the HP eradication group, the expression of cIAP2 mRNA/protein significantly decreased (p=0.006) and apoptosis increased at the 12-month follow-up after ESD. In the HP noneradication group, the aforementioned changes were not found during the same follow-up period.CONCLUSIONS:
The expression of cIAP2 increased during gastric carcinogenesis after HP infection; HP eradication in the patients who had undergone ESD for EGC reversed overexpression of cIAP2 and suppressed cell apoptosis.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Stomach Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
RNA, Messenger
/
Follow-Up Studies
/
Helicobacter pylori
/
Helicobacter
/
Apoptosis
/
Inhibitor of Apoptosis Proteins
/
Real-Time Polymerase Chain Reaction
/
Carcinogenesis
/
Gastritis, Atrophic
Type of study:
Observational study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Gut and Liver
Year:
2017
Document type:
Article