Comparative study on serum pharmacochemistry of Gardenia jasminoides under physiological and pathological status / 中草药

ABSTRACT

Objective: By means of serum pharmacochemistry combined with modern chromatographic technology to elucidate the material basis for efficacy of aqueous extract from Gardenia jasminoides (AEGJ), which has antipyretic activities with bitter-cold property. Methods: Waters 2695 HPLC was employed as the detection system, based on the same chromatographic eluted condition, physiological and DNP-induced fever syndrome serum samples, collected from hepatic portal vein of rats after ig administration of AEGJ, were under determining to compare the absorptive variation of the chemical compounds. Results: The chromatographic fingerprints of common peaks for six batches of AEGJ were established to ascertain the optimized administration dosage, blood collection time, and the method used to process the serum. Compared with the normal administrated group under physiological status of rats, we found that the significant increment absorption of jasminoidin and the generation of two new migration compounds in the DNP fever syndrom model group. Conclusion: The increment absorption and the migration of chemical compounds in serum of DNP fever syndrome pathological model of rats might reasonably lead us to consider these chemical compounds as symbols of the bitter-cold property of G jasminoides.