Effect of FTY720-P on the differentiation and maturation of MC3T3-E1 cells / 中国修复重建外科杂志
Chinese Journal of Reparative and Reconstructive Surgery
; (12): 285-290, 2018.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-856812
Responsible library:
WPRO
ABSTRACT
Methods:
The MC3T3-E1 cells were divided into the experimental group and the control group. In the experimental group, the cells were induced by the medium containing 400 ng/mL FTY720-P (chloroform as solubilizer) in vitro. In the control group, the cells were cultured with the medium only containing chloroform. The cell morphology of 2 groups were observed by inverted phase contrast microscope; the expression of osteoblast related protein (collagen type Ⅰ and collagen type Ⅲ) was detected by immunofluorescence staining; the alkaline phosphatase (ALP) staining and alizarin red staining were used to observe the formation of osteoblasts and the formation of mineralized nodules in 2 groups; and the TUNEL fluorescence assay was used to detect the cell apoptosis.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Reparative and Reconstructive Surgery
Year:
2018
Document type:
Article