Expression of Krupple-like factor 6 and inducible nitric oxide synthase in apoptosis of RAW264.7 infected by pseudomonas aeruginosa supernatant / 中国药理学通报

ABSTRACT

Aim To detect the expression of Krupple-like factor 6 ( KLF6) and inducible nitric oxide syn-thase (iNOS) in mouse mononuclear macrophage leukemia cells infected with pseudomonas aeruginosa (PA) supernatant, and to investigate the role of KLF6 protein and iNOS protein in the apoptosis of RAW264. 7 infected with PA supernatant. Methods The effect of PA supernatant on the proliferation of RAW264. 7 was detected by MTT assay. The apoptotic rate was detected by flow cytometry. The morphological changes of RAW264.7 were detected by Hoechst 33342 staining. The effect of PA supernatant on KLF6 and iNOS protein expression was detected by Western blot. Results PA supernatant inhibited the proliferation of RAW264.7 in a concentration- A nd time-depend-ent manner. PA supernatant significantly increased the percentage of RAW264.7 apoptosis and promoted the expression of KLF6 and iNOS. Conclusions PA can inhibit the proliferation of RAW264. 7, which may induce apoptosis through KLF6 and iNOS protein expres-sion.