Apoptosis related changes in the brain of diabetic mice and the effect of amyloid precursor protein 17 mer peptide / 中国药理学通报

ABSTRACT

Aim: To investigate whether the changes of neuron apoptosis related proteins exist in the brain of diabetic mice and to observe the effects of β-amyloid precursor protein (APP) 17-mer peptide on these changes. Methods: Mouse diabetic model was produced with streptozotocin, and APP17-mer peptide was injected subcutaneously as a treatment. Four weeks later, brain slides of mice were studied by immunohistochemistry for Fas, Fas-L, NF-κB, Presenilin-1 (PS-1), α-Synuclein and by TUNEL to observe the DNA fragments of neurons. Results: Circled digit one The number of neurons stained positively by Fas, Fas-L, PS-1, α-Syn antibodies in the brain of diabetic mice increased. In contrast to diabetic mice the number of positively stained hippocampal cells in normal mice were markedly reduced. The result of APP17 mer peptide-treated mice was the same as that in normal mice. The hippocampal neurons of diabetic mice showed significant intracellular decrease in NFκB. Treatment with APP 17mer peptide normalizes the expression of NFκB in diabetic mice. Circled digit two There were very little apoptotic neurons stained by TUNEL in the hippocampus of the three groups. Conclusions: The apoptosis related protein changes exist in the brain of diabetic mice. APP17mer peptide can normalize the expression of apoptosis related proteins, indicating that APP17mer peptide can improve the apoptosis abnormalities of diabetic mice and retard neuronal degeneration.