Preparation of Transferrin Modified Harmine Magnetoliposomes and Evaluation of Its Antitumor Effect / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare the transferrin modified harmine magnetoliposomes and investigate its in vitro properties and antitumor effect. METHODS: Transferrin modified harmine magnetoliposomes was prepared by reverse phase evaporation method. The size, Zeta potential, entrapment efficiency, drug loading efficiency, release rate and plasma stability of liposomes were investigated. Furthermore, cytotoxicity to liver cancer and glioma were investigated too. RESULTS: The transferrin modified harmine magnetoliposomes were successfully prepared. The average size of liposomes was (184.7±8.08)nm.The average Zeta potential was (-12.4±0.896) mV and average entrapment efficiency was (84.67±6.26)%. Drug loading efficiency was (9.14±4.54)%. Transferrin modified harmine magnetoliposomes released more slowly and were more stable in rat plasma than harmine solution. The results of cytotoxicity test showed that the cell inhibitory effect of transferrin modified harmine magnetoliposomes was enhanced compared with other groups, and the cell inhibitory effect of this preparation was more obvious underwhen cells were put under magnetic field compared with those under the non-magnetic field(P<0.05). CONCLUSION: This method can be successfully used to prepared transferrin modified harmine magnetoliposomes, and liposomes can inhibit the cell visbility of liver cancer and human glioma cells significantly.