Exploration into the Anti-Rheumatoid Arthritis Mechanism of Ethyl Acetate Extract from Kadsura longipedunculata Finet et Gagnep / 中国药学杂志

ABSTRACT

OBJECTIVE: To research the effect and mechanism of ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. METHODS: The 70 SD male rats were randomly divided into 7 groups normal control group, model group, tripterginum glycosides(TG) group, dexamethasone(DXM) group, ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. low, medium and high dosage groups(0.063, 0.126, 0.252 g·kg-1·d-1), the CIA(collagen induced arthritis) model of rats was adopted. Under 28 days intragastric administration, the rats' state, weight, degree of paw swelling, arthritis index and pathological changes of ankle joints were observed. Their serologic contents of interleukin 1β(IL-1β), interleukin-6(IL-6), and tumor necrosis factor-α(TNF-α) were examined by means of ELISA, and the expression of NF-κB p65 mRNA in joint synovial tissues were tested via quantitative Real-time PCR method. RESULTS: The ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. is found obviously effective in inhibiting CIA rats' paw swelling, decreasing serologic content of IL-1β, IL-6, and TNF-α, as well as the expression of NF-κB p65 mRNA in synovial tissues.Its effect is dosage-related, and stronger than that of TG, weaker than that of DXM. CONCLUSION: Kadsura longipedunculata Finet et Gagnep. is, to some extent, effective against RA, the mechanism of which is related to the transcription of NF-κB p65.