Rapid Serological Identification for Monophasic Salmonella Serovars with a hin Gene: Specific Polymerase Chain Reaction
Journal of Bacteriology and Virology
; : 291-298, 2005.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-85879
Responsible library:
WPRO
ABSTRACT
Salmonellosis is one of the most common food born diseases in Korea. However, it takes more than 8 days and many expensive antiserums are used for the identification of Salmonella serovars since the microorganism easily undergoes phase variation. According to the data that 65.5% of Salmonella isolates in 2000~2004 year had monophasic flagella, we have developed a rapid serological identification method using a hin gene-specific polymerase chain reaction (PCR) for monophasic Salmonella isolates that does not require the time-consuming phase conversion experiments. Using our new method, 'hin specific PCR-based serological test', we could identify serovars of monophasic Salmonella in 4 days. For the purpose of rapid identification of salmonella serovars collected from outbreaks and sporadic cases, hin specific PCR-based serological tests will be a fast and efficient method.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Salmonella
/
Salmonella Infections
/
Serologic Tests
/
Polymerase Chain Reaction
/
Disease Outbreaks
/
Flagella
/
Korea
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2005
Document type:
Article